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首页> 外文OA文献 >Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains
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Comparative Evaluation of the New Version of the INNO-LiPA Mycobacteria and GenoType Mycobacterium Assays for Identification of Mycobacterium Species from MB/BacT Liquid Cultures Artificially Inoculated with Mycobacterial Strains

机译：从人工接种分枝杆菌菌株的MB / BacT液体培养物中鉴定分枝杆菌种类的新版本INNO-LiPA分枝杆菌和基因型分枝杆菌检测方法的比较评价

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The performance of two DNA line probe assays, a new version of INNO-LiPA Mycobacteria (Innogenetics, Ghent, Belgium) and the GenoType Mycobacterium (Hain Diagnostika, Nehren, Germany), were evaluated for identification of mycobacterial species isolated from liquid cultures. Both tests are based on a PCR technique and designed for simultaneous identification of different mycobacterial species by reverse hybridization and line probe technology. The INNO-LiPA Mycobacteria v2 targeting the 16S-23S rRNA gene spacer region was developed for the simultaneous identification of 16 different mycobacterial species. The GenoType Mycobacterium, which targets the 23S rRNA gene, allows simultaneous identification of 13 mycobacterial species. Both tests were evaluated on 110 mycobacterial strains belonging to 22 different mycobacterial species (20 reference strains, 83 clinical strains, and 4 Mycobacterium kansasii strains isolated from tap water) that were previously inoculated into MB/BacT bottles. The sensitivity of both methods, defined as the number of positive results obtained with the Mycobacterium genus probe together with an interpretable result on the number of samples tested was 110 of 110 (100%) for INNO-LiPA and 102 of 110 (92.7%) for GenoType. For samples with interpretable results, INNO-LiPA was able to correctly identify 109 of 110 samples (99.1%), whereas the GenoType correctly identified 100 of 102 samples (98.0%). Both tests were easy to perform, rapid, and reliable when applied to mycobacterial identification directly from MB/BacT bottles.
机译：为了鉴定从液体培养物中分离出的分枝杆菌菌种，评估了两种DNA线探针测定法的性能，即新版的INNO-LiPA分枝杆菌（Innogenetics，比利时根特）和GenoType分枝杆菌（Hain Diagnostika，德国内伦）。两种测试均基于PCR技术，旨在通过反向杂交和线探针技术同时鉴定不同的分枝杆菌菌种。开发了针对16S-23S rRNA基因间隔区的INNO-LiPA分枝杆菌v2，用于同时鉴定16种不同的分枝杆菌物种。靶向23S rRNA基因的GenoType分枝杆菌可以同时鉴定13种分枝杆菌。两项测试均对先前已接种到MB / BacT瓶中的属于22种不同分枝杆菌物种的110种分枝杆菌菌株（20种参考菌株，83种临床菌株和4种自自来水分离的堪萨斯分枝杆菌菌株）进行了评估。两种方法的灵敏度均定义为分枝杆菌属探针获得的阳性结果的数量以及所测试样品数量的可解释结果，其中INNO-LiPA的灵敏度为110的110（100％），110的灵敏度为102（92.7％）对于GenoType。对于具有可解释结果的样品，INNO-LiPA能够正确识别110个样品中的109个（99.1％），而GenoType可以正确识别102个样品中的100个（98.0％）。直接用于MB / BacT瓶中的分枝杆菌鉴定时，两种测试均易于执行，快速且可靠。

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